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Additionally, integrated transgenic strains were outcrossed to the N2 wild-type strain a minimum of three times to remove background mutations and ensure genetic consistency

3 Preparation of cell models of GPX4 R152H

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Br J Pharmacol 172:16331637 Wallace JL, Blackler RW, Chan MV, Da Silva GJ, Elsheikh W, Flannigan KL, Gamaniek I, Manko A, Wang L, Motta JP, Buret AG (2015) Anti-inflammatory and cytoprotective actions of hydrogen sulfide: translation to therapeutics

In dermal and corneal models, Tb4 accelerates re-epithelialization (Malinda et al., J Invest Dermatol , 1999