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Following synthesis, the crude peptide undergoes purification using reverse-phase high-performance liquid chromatography (RP-HPLC) to remove synthesis-related impurities and isolate the target compound

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Always escalate one compound at a time with at least a two-week stabilization period between escalations

Bound peptides were washed with 5% ACN, 0.1% TFA and eluted with 50% ACN, 0.1% TFA

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