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Two gut microbiota inocula from the feces of high-TMAO producers were prepared by mixing 1 g of feces with 15 mL of PBS with 0.05% cysteine and filtering the mixture through a 100-m Falcon cell strainer, following which 100 L of the stool filtrate was transferred to 1.9 mL of medium and subsequently incubated at 37 C under anaerobic conditions (80% N 2 , 10% CO 2 , and 10% H 2 ) for 0, 6, 12, and 24 h

However, the really valuable ingredients are the bacosides, which belong to the saponins

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exceeding this limit provides no extra benefit, as the excess is excreted in urine