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RT-qPCR was carried out by the 2SYBR Green Master Mix (Abclonal, RK21203)

2), including serotonin, melatonin, kynurenine, and quinolinic acid (QUIN)

Knapp and Klann, 2002)

Protein degradation assay Raw264.7 cells were cultured in six-well plates at the density of 3 10 5 cells/well

Staying flexible and keeping an open line of communication with your healthcare provider will ensure you stay on the right path

Therefore, only rats that were unresponsive lost their righting reflex and whose pupils were dilated during ischemia were selected to evaluate completed cerebral ischemia, as recommended by Pulsinelli (Pulsinelli et al