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Membranes were probed with antibodies against MCT1 (sc-365501, 1:200 dilution, Santa Cruz Biotechnology, CA, USA) or MCT4 (sc-50329, 1:500 dilution, Santa Cruz Biotechnology, CA, USA) in 0.5% w/v milk in PBS (4 C, overnight incubation)

Although the basic cobalamin molecule is only synthesized by microorganisms, all mammalian cells can convert this into the coenzymes adenosylcobalamin and methylcobalamin

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The information on the relevant siRNA sequence is provided below

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