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Consistently, another group recently reported that USP29 promotes aerobic glycolysis via stabilizing HIF1 to mediate sorafenib resistance in HCC cell lines, suggesting that USP29 may play a key role in the regulation of aerobic glycolysis in different cancer types [34]

For this purpose, according to the kits instruction, a cell suspension with a dilution of 1 million cells/ml was prepared in the phosphate-buffered saline (PBS) at pH 7.2-7.4

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These observations were further confirmed by Hoechst 33342 staining, which revealed extensive detachment of cells from the cell culture substratum and increases in apoptotic bodies of HepG2 cells incubated with H 2 O 2 and TNF for 16 h (Fig