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These lipid aldehyde adducts can subsequently interfere with protein function in a number of ways including the induction of protein crosslinking, structural perturbation and protein aggregation 52,53,54,55,56

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By using the ability of puromycin to incorporate into protein during synthesis and our ability to detect it with an anti-puromycin antibody, the SCENITH method utilizes the level of puromycin incorporation as a marker of protein synthesis and thus, a surrogate marker of cell global metabolic activity (Figure 2B)

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The lack of conserved markers hampers clinical assessment and underscores the need for human-centric validation pipelines that integrate single-cell transcriptomic and imaging datasets with body fluid biomarkers