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Transcriptome analysis of Capsicum chlorosis virus-induced hypersensitive resistance response in Bell capsicum

Synthesis of ROS-sensitive polymers (P3) To a solution of ROS sensitive compound (2,2-(propane-2,2-diylbis(sulfanediyl))bis(ehan-1-ol)) (0.26 g, 1.35 mmol) in anhydrous DMF (5 ml), 1,2,4,5-cyclohexanetetracarboxylic dianhydride (0.31 g, 1.41 mmol) was quickly added into the solution

Rossi et al., 2006

HEK293T cells were grown to 70% confluency in 96-well plates (Corning) at 37C in DMEM supplemented with 10% FBS, and treated with Entranster TM -H (Engreen) transfection reagent (0.1 L) and purified plasmids [0.25 g pGL4.74 (hRluc/TK) and 0.25 g pGL4.32(luc2P/NF-B-RE/Hygro plasmid)] in 50 L DMEM/10% FBS per well

These strategies have only been used in few thiol-based agents

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