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2.8 Measurement of nucleoside triphosphates Nucleotides were extracted from 4 to 5 10 6 cell pellets using perchloric acid, and the extracts were neutralized with potassium hydroxide

Belsky, D.W., et al

doi: 10.1097/WNN.0000000000000189, 202

The formation of is an irreversible process catalyzed by pseudouridine synthases (PUS) via two distinct pathways: a protein-only mechanism mediated by PUS enzymes and an RNA-dependent mechanism involving box H/ACA ncRNAs and the Cbf5/dyskerin complex [103]

Collectively, NNT is therefore a key enzyme bridging cell metabolism and reductive stress

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