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Mushroom hardening can be related to the air pockets inside the mushroom released during the blanching process and replaced by the tissue (Jaboska-Ry et al., 2019a, 2019b, 2019c, 2019d)

Fibrinogen aptamer (5-NH 2 -GGTTGGTGTGGTTGG-3) was procured from Ponsure Co., Ltd

Montalban-Bravo G, DiNardo CD

Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol