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For IL-1 treatment, cells were incubated in plain Dulbeccos modified Eagles medium (DMEM):F12 medium containing 6 mM glucose and 2 mM glutamine overnight followed by stimulation with IL-1 (10 ng/ml) or 0.1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) (vehicle control)

There are also specific alterations in T cell subsets associated with aging

Cross-immunity with Haemaphysalis longicornis glutathione S-transferase reduces an experimental Rhipicephalus ( Boophilus ) microplus infestation

Consistent with our observations, CYP9Q2 and CYP9Q3 are known to be constitutively expressed at low levels in bees 17

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