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Next, cell suspensions were prepared and blocked in a 0.3% bovine serum albumin (BSA) solution, incubated with primary antibodies (including PE-CD86, APC-CD206, and FITC-CD11b

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The focus of preclinical studies has shifted from investigating glucose metabolism alone to exploring more complex mechanisms, such as the metabolic interplay between Schwann cells and axons and the role of insulin resistance in nerve function

This methodology is able to detect relevant biological features across different molecular layers and how they interact with each other by retaining complex patterns from entire datasets [27]

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