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Additionally, the levels of SOD and GPx, which are part of the enzymatic antioxidant system, showed an increase 74

Elekwa I, Monanu M, Anosike E

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Blood samples were collected from the cows (10 ml) before injection of micronutrients on 30 th , 15 th , 7 th days before the expected date of parturition, on the day of parturition and on days 7 th , 15 th and 30 th after parturition and in calves, 6 ml of blood were collected from each newborn calves at birth i.e., day 0 and on days 1, 2, 3, 4, 7, 8, 15, 30 and 45 post-calving via jugular vein puncture in sterile K2 EDTA vacutainer tubes (Greiner bio-one, Vacuette, Austria) for neutrophils, lymphocytes isolation and plasma separation