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Serum estrogen, progesterone, and insulin levels were measured using the Ultrasensitive Estradiol kit (Beckman Coulter, Brea, CA), Progesterone EIA kit (Cayman Chemicals, Ann Arbor, MI), and mouse insulin ELISA (Millipore, Inc., Burlington, MA), respectively, following the manufacturers protocols

Reactive astrocytes exacerbate inflammation by releasing additional cytokines, contributing to a feedback loop that worsens neuronal injury ( The interaction between excitotoxicity and neuroinflammation is critical for the development of long-term neurodegeneration

The evidence base for systemic human applications, however, is not yet at the level required to make confident clinical recommendations which is precisely why the research continues

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