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In contrast, KRAS G13D cells exhibited a distinct and more sensitive response to HER2 suppression

Measurement of ROS production To measure intracellular ROS, the 143B cells were plated in 12-well plates at a density of 3 10 5 cells per well

Eur J Pediatr

The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat