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Immunofluorescence staining was performed to assess macrophage polarization, with CD86 serving as a marker for pro-inflammatory M1 macrophages and CD206 for anti-inflammatory M2 macrophages

The CES-D-8 was introduced in the TILDA study at waves 2 and 3, and a score of 9 on this scale was used to define clinically significant depressive symptoms at these waves (Reference Briggs, Carey and Kenny32,Reference OHalloran, Kenny and King-Kallimanis33)

Seveg G, Fascetti AJ, Weeth LP, Cowgill LD (2010)

Though promising, the available efficacy data are from isolated case reports.78 Neuromodulation

Q4: What is the difference between the treatment-policy and trial-product estimands

Smallscale raisers also face the same biological problems, only with fewer heads