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The acidic and reducing environment in lysosomes results in the formation of ferrous (Fe 2+ ) species, which in turn may catalyze the decomposition of H 2 O 2 in the Fenton reaction and produce OH radicals

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Chronic elevation at 12% significantly increases the risk of long-term microvascular complications , such as retinopathy, nephropathy, and neuropathy

Target proteins were further purified by size exclusion chromatography using a Superdex 75 16/600 column (GE Healthcare) in 20 mM Tris pH 7.5, 250 mM KCl, 1 mM DTT