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Mitochondrial Ca 2+ Uptake/Retention Capacity Mitochondrial calcium uptake/release was measured by following the fluorescence changes in the medium containing 0.1 M calcium green-5N hexapotassium salt using a spectrofluorometer (Hitachi F-4500, Japan) operating at 506 nm for excitation and 531 nm for emission (Ronchi et al., 2013)

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Disruption of this barrier permits microbial translocation into the submucosa, triggering inflammatory cascades that contribute to the pathogenesis of intestinal disorders such as IBD (159)

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