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Clin Nutr ESPEN 2018;23:171-83

doi: 10.1007/s00726-013-1481-5

the additional time and the high cost associated with proteases needed for tag removal have greatly hampered the usefulness of affinity tags in large-scale industrial processes, as no simple and low-cost method exists to date for affinity tag removal

[DOI] [PMC free article] [PubMed] [Google Scholar] 8.Blyth F.M

Following internalization, the receptor is retained and recycled to the plasma membrane as a resensitized receptor or, upon recognition of an interaction with the GPCR-associated sorting protein (GASP), the receptor will be sent to the lysosome for degradation (33,43)