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Downstream processing, including gentle harvesting (centrifugation or filtration) and freeze-drying, preserves therapeutic compounds but increases costs (50100/kg), while advanced extraction (chromatography, ultrafiltration) further elevate expenses but yield high-value extracts (phycocyanin at 100500/g) (Gao et al

In both human cell cultures and mouse hippocampal slices, it was found that acute treatment with the CB 1 agonist WIN 55,212- mesylate caused G/-mediated inhibition of Ca 2+ channels, which decreased the vesicular release of the neurotransmitter and also inhibited adenylyl cyclase, resulting in a decrease in cAMP levels [62]

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This gap prevents dose-response relationships from animals from being translated into safe and effective design of human doses for fertility

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