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Fluorescence in situ Hybridization (FISH) FISH was performed as previously described ( 2 O 2 in ethanol for 2 h and then hybridized overnight in hybridization buffer (20 mM TrisHCl pH 8.0, 0.9 M NaCl, 0.01% wt/vol sodium dodecyl sulfate, 30% vol/vol formamide) containing 10 pmol fluorescent probe/mL

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However, longer polymethine chain length increases its flexibility, thus increasing the non-radiative dissipation of the excited state energy and reducing the triplet state quantum yield (critical for PDT) 178 , although increasing the rigidity of polymethinium chain cannot always lead to an increase in the photodynamic efficiency 180

Epigenetic modulation of BRCA1 and BRCA2 gene expression by equol in breast cancer cell lines