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The PC family of convertases are all synthesized as inactive precursors with a lengthy N-terminal propeptide that is autocatalytically cleaved at a tetrabasic cleavage site by the proenzyme as it passes from the slightly alkaline ER to the neutral and mildly acidic conditions of the Golgi apparatus and is then removed by a second cleavage within the propeptide which assists in its release and disposal, yielding the active full-length enzyme

Cells were disrupted using an ultrasonic system

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This is especially difficult because under IL-13 conditions, upregulation of additional factors, including GPX4 and SLC7A11 (9), occur in an effort to maintain intracellular homeostasis

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Cell Host Microbe 28(2):306312.e6 Zheng X et al (2022) N-acetyltransferase 10 promotes colon cancer progression by inhibiting ferroptosis through N4-acetylation and stabilization of ferroptosis suppressor protein 1 (FSP1) mRNA