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The lv-shRNAs targeting NF1 were synthesized according to the sequences of si-NF1-1 and si-NF2-1 and inserted into the GV493 vector

10.1016/j.chom.2015.01.001 94

Ganassi, M

Biochemical tools for characterizing individual fungal proteins and genetically manipulating fungal strains have significantly advanced our understanding of enzyme function and central carbon metabolism (i.e., glycolysis/gluconeogenesis, pentose phosphate pathway or PPP, TCA cycle, and transhydrogenase cycle)