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Michalon et al., 2014

Despite the differences in the microbial composition and metabolic potential of the different GIT niches, all showed similar changes toward carbohydrate metabolism

Lentivirus was produced by transfecting HEK293T cells plated in a T75 flask with 6 g of psPAX2, 3 g of pMD2.G and 12 g of lentiviral vector containing the LaccID, TCR or CAR with 60 l of PEI Max (1 mg ml 1 in water, pH 7.3)