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using a higher volume produces a more dilute solution, which may be easier to measure accurately

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Lifestyle as Medicine What Tuell and colleagues highlight is both reassuring and empowering: glutathione is not fixed

After transient transfection in HEK cells, WT nrf2a strongly activates the transcription of the firefly luciferase reporter gene, whereas both the nrf2a5 and nrf2a fh318 mutants induced much weaker expression of the reporter gene (Fig 1B)