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Aslani M, Mortazavi-Jahromi SS, Mirshafiey A

recently investigated dihydronicotinamide riboside (NRH) and demonstrated its potency as a pharmacological agent for enhancing NAD + concentrations in mammalian cells (Yang et al

For analysis of PhoA accumulation, 1 mL of each aforementioned cultures were harvested and the pellet was resuspended in standard 1x SDS-sample buffer adjusted to 100 L for an OD 600 of 1.0