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10.3390/biomedicines8110446 116 TuH

People with kidney disease should avoid high-dose cyanocobalamin supplements specifically, as there is evidence it may accelerate kidney function decline

Measurement of cellular iron Cell iron content assay kit (Solarbio, Beijing, China) was used to measure cellular iron contents based on assay contains hydroxylamine hydrochloride, which reduces Fe 3+ to Fe 2+ in cells, and under weak acid conditions, Fe 2+ and phenoline form a reddish-orange mixture, and the iron content can be calculated by measuring the OD value at 510 nm

Acknowledgments UF thank the Indian Council of Medical Research-Department of Health Research (ICMR-DHR) for the award of Young Scientist

Their age ranged from 6 months to 3 years

GraphPad Software, San Diego, USA) was used to create the figures