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To test if the inducible degradation of Vma2 protein affects vacuolar acidification, we fused the C terminus of Pho8, a vacuolar-resident protein with the C terminus facing the lumen, with a pH-sensitive super ecliptic pHluorin (SEP) protein that displays increased fluorescence with higher pH levels 27

These include hyperpigmentationor excess melanin around the eyesand visible blood vessels