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They were washed with and resuspended in PCA medium (5 mM MES pH 5.8, 0.32% Gamborgs B5 basal salt powder with vitamins, 2 mM MgSO 4 .7H 2 O, 3.4 mM CaCl 2 .2H 2 O, 0.342 mM l-glutamine, 58.4 mM sucrose, 444 mM glucose, 8.4 M calcium pantothenate, 4 g/ml biotin, 64.52 g/l ampicillin) at ~300,000 protoplasts per 1.8 ml, before immobilization in 0.75% (w/v) agarose (see below) to acquire confocal microscopy images

Similarly the invention relates to methods for the treatment and/or prevention of a CNS disorder, wherein an effective dose of a conjugate of the invention is administered to a subject in need thereof

Binders such as activated charcoal, zeolite, bentonite clay, silica, citrus pectin, chlorella, or chitosan are used to catch toxins in the gut during Phase 3

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The test compound was pre-incubated with purified human recombinant cyclooxygenase 1 (COX-1) or cyclooxygenase 2 (COX-2) (Cayman Chemical) for 15 min