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siRNA-mediated RNA Interference For RNA interference, cells in 6-well plates were transfected with designed siRNAs (an irrelevant sequence [control] siRNA), GSTP1 siRNA-1 and GSTP1 siRNA-2, each at 5 nmol/L, using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) for 48 h according to the manufacturers instructions

To improve male infertility care, it is recommended to practice an integrate test for heavy metals, phthalates, bisphenol A (BPA), and microplastics into standard infertility evaluations, particularly for unexplained low or absent sperm counts

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Cryopreservation involves the surgical removal of ovarian tissue via laparoscopy or laparotomy, excluding the medulla while retaining the cortex, which is then sectioned into slices approximately 1 mm thick and 510 mm in length and width