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(Tokyo, Japan) and raised in the Division of Laboratory Animal Research, Research Center for Support of Advanced Science, Shinshu University, under specific pathogen-free (SPF) conditions at 24 2 C with a light-controlled regimen (12 hours light/dark cycle)

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IGF1R deficiency reduces tumor growth, proliferation, inflammation and vascularization, and increases apoptosis after tumor heterotopic transplantation Since IGF1R has been implicated in the pathogenesis of lung cancer by facilitating metastasis, tumor-associated inflammation and immune checkpoint regulation [12, 16], we decided to assess whether IGF1R deficiency in the TME delays implantation and progression in a heterotopic LLC mouse model