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MDM2 upregulation induces mitophagy deficiency via Mic60 ubiquitination in fetal microglial inflammation and consequently neuronal DNA damage caused by exposure to ZnO-NPs during pregnancy

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Postnatal cortical neuron isolation and culture Postnatal cortical neurons were isolated following a protocol adapted from Brewer and Torricelli (2007) and the Miltenyi Biotec MACS protocol for the isolation and cultivation of neurons from adult mouse brain

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