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Antioxidant activity is quantified by the reduction in area under the ethylene concentration-time curve relative to the radical-only control ( 3.1.2 Chemiluminescence quenching Chemiluminescent assays quantify antioxidant activity by measuring the attenuation of chemiluminescence generated through reactions between ROS and specific probes such as luminol or lucigenin

Then, cells were incubated with PE-conjugated anti-mouse CD86 or CD206 antibodies (2 L/test, BioLegend) for 30 min at 4 C in darkness

Determination of midpoint potentials of roGFP variants Both roGFP2-iL and GRX-roGFP2-iL (1 M final concentration) were allowed to equilibrate (23 h) with lipoic acid buffers [reduced form dihydrolipoic acid (DHLA)

Much of the homocysteine must be recycled back into methionine, and this is where B12 (in the form of methylcobalamin), METHYL-THF, and methionine synthase (which allows us to convert to methionine) come into play

Activity assays of mammalian thioredoxin and thioredoxin reductase: fluorescent disulfide substrates, mechanisms, and use with tissue samples

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