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Intellia started dosing patients in their phase I/II trial in 2020

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doi: 10.1021/acsnano.6b08731 189 ShiYGongMDengZLiuHChangYYangZet al

Nucleases that induce double-stranded DNA breaks, such as ZFN and TALEN, can used to engineer knockin mice

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Samples to undergo reductive amination were dissolved in 15l200 mM NaOAc buffer pH 5.0 5 ul specific BB was added to each well (200 mM in DMSO) and incubate at 30C for 1 h