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The post-treatment follow-up was performed after 12 and 24 weeks of treatment, plus a few days if necessary, so that patients were again evaluated on day 36 of the menstrual cycle (the first occurring after the 12 or 24 weeks of treatment)

Similarly, for overexpression cell lines, gBlocks (IDT) containing the cDNA of interest were cloned into pMXS-IRES-Blast linearized with BamHI and NotI by Gibson Assembly (NEB)

Arginine is also at the center of the different functions associated with M1 and M2 macrophages, being called a small amino acid for macrophage metabolism, a giant controller for mammalian pathophysiology [8]