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Furthermore, when FLAG-UBQLN2 was transiently overexpressed in -syn(WT)-EGFP/SH-SY5Y cells, UBQLN2 formed puncta, as detected by anti-FLAG staining

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In a rat IVDD model, disc height recovered by 87%, and Modic changes were reversed, addressing the mechanical and anti-fibrotic challenges posed by late-stage ECM stiffening (Tao et al., 2022)

Western blotting After the samples were resolved by SDS-PAGE and transferred to a nitrocellulose membrane, total proteins were visualized with Ponceau S staining

Indeed, post exposure treatment with atropine methyl bromide, which does not cross the blood-brain barrier, resulted in 100% survival of VX-exposed animals