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Similar to Caspase-1, expression of Caspase-8, which we and others have documented to process IL-1 28,29,30,31,32 , also resulted in WT IL-1 and IL-1 K133R secretion, and this was reduced upon expression of the IL-1 133 mutant that abolishes the K133:D129 electrostatic interaction (Fig

This study had three limitations

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The labeled fractions of proline M2 and arginine M1 in tumors were approximately 0.02 and 0.01, respectively, confirming the presence of active glutamate metabolism (Supplementary Fig