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Zhang H, Huang C, Gu X, et al

The Neh2 domain is a regulatory domain containing the DLG and ETGE binding motifs, through which NRF2 interacts with its major negative regulator, KEAP1, and is essential for the KEAP1-mediated degradation of the protein by the proteasome [128, 143,144,145]

Next, the fixed tissues were dehydrated in gradient ethanol (70, 75, 85, 90, 95, and 100%), hyalinized in xylene, and embedded in paraffin wax at 55 C