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2013;3:e309

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The use of defined novel skeleton structures whose synthesis can be scaled up in preparation for hit validation, along with optimized downstream reactions with commercially available building blocks, will facilitate the process of hit validation

Cells were then washed twice in NB medium, counted, resuspended in NB media [Neurobasal (NB) medium: Neurobasal (21103049, Life Technologies), 1 N2 supplement, 1 B27 supplement, 1 Glutamax, 1 NEAA, 1 penicillin-streptomycin, 3.2 mg/ml glucose and 0.2 mM ascorbic acid]

You can also talk to your healthcare provider or pharmacist, who may be aware of other ways to reduce your costs