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2.1 Precise Control of Core Fermentation Parameters We have established optimal control ranges for key fermentation parameters, laying a solid foundation for stable, large-scale production: Carbon source concentration (glucose): 2228 g/L Magnesium ion concentration (magnesium sulfate): 2.03.0 g/L Culture temperature: 2630C Through continuous fermentation monitoring, we determined a 24-hour cycle as the optimal fermentation period

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The cells incorporate cysteine and most of the intracellular cysteine is incorporated into the synthesis of GSH