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Briefly, confluent cells were serum starved overnight, followed by administration of 100 nM insulin for 5 hours

Fluorescent signals of alive and dead cells were measured with a fluorometer (FLUOstar Galaxy, BMG Lab technologies, Offenburg, Germany) and the ratio between live and dead cells was calculated

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[DOI] [PMC free article] [PubMed] [Google Scholar] 64.Bagshaw SM, Al-Khafaji A, Artigas A, Davison D, Haase M, Lissauer M, et al

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DMF treatment reduced this concentration in CSF by 40% (Fig