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Curr Psychiatry 16(2):1520 Piomelli D, Astarita G, Rapaka R (2007) A neuroscientists guide to lipidomics

Thus, it can be hypothesized that temporal changes in net proteolytic activity in human milk contribute to the FAA dynamics, although this is unlikely as levels of all major human milk proteases and anti-proteases decrease during lactation, along with levels of their substrates (50, 58)

The proteins were electrophoretically transferred onto polyvinilidenedifluoride (PVDF) membranes (Bio-Rad, Hercules, California, USA) and probed with: anti-LC3 antibody (MBL), anti-P62 antibody (Sigma-Aldrich) or rabbit monoclonal anti-caspase 3 antibody (Invitrogen, Hillsboro, Oregon)

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