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Ninety six 28 days weaned piglets randomly assigned to four groups: a control group, LPS-treated groups (injection on the 15th day), LPS + 1 g/kg garlic essential oil in feed (LPS + GO, lasting for 18 days), and LPS + 5 mg/kg cefalexn in feed (LPS + CE, medication on the 15th day, lasting for 3 days), 4 pens, 6 piglets per pen, three males and three females

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A: It depends on the specific peptide

doi: 10.1080/15592294.2015.1088630

The column was washed with 6 bed volumes of wash buffer (50 mM sodium phosphate, 300 mM NaCl and 100 mM imidazole, pH 7.4) and the target protein was eluted with 6 bed volumes of elution buffer (50 mM sodium phosphate, 300 mM NaCl and 300 mM imidazole, pH 7.4)