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Description

Purification of the C-terminally Flag epitope tagged was performed by absorption of secreted protein to M2-agarose beads (Sigma), and elution by excess 3 Flag peptides (Sigma)

200 l of bacterial culture was then added to the wells of 96-well plates (Corning Corp

178 Shannons diversity index was significantly lower when comparing the bacterial community of subjects in the emphysema-only group with those of lung cancer patients