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However, due to the potential for injury to the sciatic nerve, the ventrogluteal muscle is most often used instead

Additional plateable PHHs Hum180931 (Lonza, catalog: HUCPI) were thawed and plated in hepatocyte plating medium (Lonza, catalog: MP250) overnight, then the medium was replaced with hepatocyte culture medium (Lonza, catalog: CC-3198)

That would be enough to inject a single large joint like a knee, hip, or shoulder

Splenocytes from mice vaccinated with BCG, metE DNA, or mmpL12 DNA showed statistically significant inhibition of mycobacterial growth compared to those from nave mice ( Figures 4A, B )