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Elucidating the cellular determinants of targeted membrane protein degradation by lysosome-targeting chimeras

doi: 10.1152/jn.01227.2005 242 MackieKLaiYWestenbroekRMitchellR

Add the sterile diluent (bacteriostatic water at a typical 2 mL volume for cell-culture protocols, or sterile saline for vascular research assays) directly to the lyophilized vial under aseptic technique

Balancing of terminal crtZ , crtW and crtZW expression results in biosynthesis of astaxanthin as major carotenoid The two identified pathway engineering strategies, namely an improved precursor supply and an optimized terminal fusion protein, were combined in strain BETA6 (pSH1-crtZ ~ m ~ W) (Fig