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Ischemia-Reperfusion Research: Examination of metabolic adaptations during ischemia, reperfusion injury mechanisms, and cardioprotective interventions

reconstituted 2-8C, 4 weeks Simple, deliberate steps not a protocol

Louis, 2006, Elsevier

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Under conditions of oxidative stress, the GSH/GSSG ratio decreases, indicating an increased demand for glutathione to scavenge ROS

The supernatant was mixed with an equal volume of Griess reagent [1% (w/v) sulfanilamide, 5% (v/v) phosphoric acid, and 0.1% (w/v) N-(1-Naphthyl) ethylenediamine dihydrochloride] and incubated for 10 min