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The LCQ spectrometer, controlled by the Xcalibur software (ThermoElectron, San Jose, CA), was operated in positive ion mode, since vicine, convicine and their aglycones could be detected as mono-protonated ions ([M+H] + ) at the low pH (about 4) recreated in the chromatographic mobile phase through formic acid addition

Concurrent with these cardiovascular changes, pregnancy is marked by a significant increase in plasma volume (around 3.5 L) at the end of gestation (Qasqas et al