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10.1111/j.1600-0757.2010.00377.x Periodontology 53 GuravA

For the immunofluorescence reaction, sections were rinsed in KPBS and incubated for 90 min in AlexaFluor 488 -conjugated secondary antibody (1:500, Jackson Laboratories)

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Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue

They are significantly less water soluble than comparable acids or alcohols due to: Glutamine is one of the 20 amino acids encoded by the standard genetic code

P75] = 367 [353