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Samples were run on NuPAGE 412% Bis-Tris gels using MOPS running buffer (Invitrogen)

From another point, the precise role of autoantibodies against -cell autoantigens, e.g., insulin-specific autoantibodies, insulinoma antigen, zinc transporter-8, and glutamic acid decarboxylase, in the pathogenesis of T1D remains unclear